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anti disc large dlg mouse monoclonal antibody  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank anti disc large dlg mouse monoclonal antibody
    Anti Disc Large Dlg Mouse Monoclonal Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+dlg/anti-Discs+large/pm42104039-322-18-27
    Average 96 stars, based on 44 article reviews
    anti disc large dlg mouse monoclonal antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Somatic function of the Argonaute protein Aubergine is essential for neuromuscular development and function in Drosophila
    Article Snippet: The primary antibodies used were: rabbit anti-aub, 1:1000 (see above), rat anti-aub, 1:2000 (see above), rabbit anti-Copia Pol (see above) 1:1000 [ ], rabbit anti-Copia gag , 1:5000 [ ], rabbit anti-dArc1, 1:500 [ ], rabbit anti-DLG, 1:40,000 [ ], mouse anti-DLG, 1:200 (Developmental Studies Hybridoma Bank (DSHB), 4F3) and mouse anti-GFP, 1:500 (Developmental Studies Hybridoma Bank (DSHB), 4C9).

    Article Title: Somatic cells compartmentalise their metabolism to sustain germ cell survival
    Article Snippet: Williams) and mouse anti-Dlg (4F3, 1:20, deposited by C. Goodman) were obtained from the Developmental Studies Hybridoma Bank created by the NICHD of the NIH and maintained at The University of Iowa.

    Article Title: Drosophila ryanodine receptor gene triggers functional and developmental muscle properties and could be used to assess the impact of human RYR1 mutations
    Article Snippet: The following primary antibodies were used in this study: guinea pig anti-dRYR antibody (1:200, kindly provided by Robert Scott from NIH/NIMH Institute), mouse anti-Dlg (1-50, DSHB, 4F3), rat anti-actin (1-500, Abcam, ab 50591), mouse anti-kettin (1:50 DSHB 1B8-3D9), mouse anti-ATP5A (1:200 Abcam, ab 14748), goat anti-GFP (1:500, Abcam, ab 5450), and chicken anti-β galactosidase (1:1000, Abcam, ab 9361).

    Article Title: The cell autonomous and non-autonomous functions of Rab27 in longevity and neuroprotection in Drosophila
    Article Snippet: Mouse anti-dlg (discs large) , Developmental Studies Hybridoma Bank , 4F3 RRID: AB_528203.

    Affinity Purification:

    Article Title: Mushroom bodies tiny regulates Sidekick localization to tricellular adherens junctions
    Article Snippet: Embryos were washed in PBST and mounted in Fluoromount-G (Southern Biotech). .. Antibodies used were chicken anti-GFP (ThermoFisher A10262, RRID AB_2534023, 1:400), guinea pig anti-Sdk ( Astigarraga et al., 2018 ) (affinity-purified serum used at 1:200 or unpurified serum used at 1:4000), mouse anti-Arm (Developmental Studies Hybridoma Bank (DSHB) N2 7A1, RRID AB_528089 1:20), rat anti-Ecad (DSHB DCAD2, RRID AB_528120, 1:20), rabbit anti-DsRed (Takara Bio 632496, 1:500), guinea pig anti-Mbt ( Walther et al., 2016 ) (1:200), rat anti-NCad (DSHB DN-Ex #8, RRID AB_528121, 1:20), rat anti-Elav (DSHB Rat-Elav-7E8A10, RRID AB_528218, 1:100), mouse anti-Dlg (DSHB 4F3, RRID AB_528203) and mouse anti-Pyd (DSHB PYD2, RRID AB_2618043, 1:10). .. Secondary antibodies were from Invitrogen (Alexa488-conjugated, 1:1000) or Jackson ImmunoResearch (Cy3- or Cy5-conjugated, 1:200).

    Incubation:

    Article Title: Interaction between neuromuscular junction metabolic requirements in fragile X syndrome and glycogen storage disease models
    Article Snippet: .. Preparations were incubated overnight at 4°C in mouse anti-DLG (DSHB, 4F3, 1:100), washed 3× with PBS-TX for 10 min each, then incubated for 4 h at RT in Alexa Fluor 488-conjugated goat anti-mouse-IgG (Thermo Fisher Scientific, A-11001, 1:300) and Alexa FluorCy3-conjugated goat anti-HRP (Jackson ImmunoResearch, 123-605-021, 1:300). ..

    Article Title: Functional profiling and visualization of the sphingolipid metabolic network in vivo
    Article Snippet: .. The brains were permeabilized with 2% PBST for 30 min, then blocked in 0.25% PBST with 1% BSA for 1 h. Next, the samples were incubated with primary antibodies, rat anti-Elav (1:200, Rat-Elav-7E8A10, DSHB), mouse anti-Repo (1:200, 8D12 anti-Repo, DSHB), mouse anti-lamin (1:200, ADL67.10, DSHB), mouse anti-ATP5A (1:500, ab14748, Abcam), mouse anti-Cathepsin L (1:500, MAB22591, R and D Systems), mouse anti-Na + /K + -ATPase (1:200, a5, DSHB), rabbit anti-Ref2P (1:200, ab178440, abcam), mouse anti-DLG (1:200, 4F3 anti-discs large, DSHB), rabbit anti-GM130 (1:200, ab30637, Abcam), goat anti-Golgin245 (1:500, Golgin245, DSHB), mouse anti-Cnx99A (1:200, Cnx99A 6-2-1, DSHB), mouse anti-Golgin84 (1:200, Golgin84 12-1, DSHB), chicken anti-GFP (1:500, ab13970, Abcam), rabbit anti-Ifc (1:200, Jung et al, ) rabbit anti-Calnexin (1:200, ab75801, Abcam), rabbit anti-rab5 (1:200, ab31261, Abcam), diluted in 0.25% PBST with 1% BSA overnight at 4 °C, washed in 1% PBST four times for 30 min, and incubated in secondary antibody (1:500, 111-605-003, 715-545-150, 711-545-152, 112-545-167, 111-165-003, 115-605-166, Jackson ImmunoResearch Laboratories) diluted in 0.25% PBST with 1% BSA overnight at 4 °C. .. The brains were then washed with 1% PBST four times for 30 min and mounted in Vectashield (H-1000, Vector Laboratories) or RapiClear 1.47 (#RC147002, SunJin Lab) for subsequent confocal imaging.

    Article Title: Functional profiling and visualization of the sphingolipid metabolic network in vivo.
    Article Snippet: .. The brains were permeabilized with 2% PBST for 30 min, then blocked in 0.25% PBST with 1% BSA for 1 h. Next, the samples were incubated with primary antibodies, rat anti-Elav (1:200, Rat-Elav-7E8A10, DSHB), mouse anti-Repo (1:200, 8D12 anti-Repo, DSHB), mouse antilamin (1:200, ADL67.10, DSHB), mouse anti-ATP5A (1:500, ab14748, Abcam), mouse anti-Cathepsin L (1:500, MAB22591, R and D Systems), mouse anti-Na+/K+-ATPase (1:200, a5, DSHB), rabbit anti-Ref2P (1:200, ab178440, abcam), mouse anti-DLG (1:200, 4F3 anti-discs large, DSHB), rabbit anti-GM130 (1:200, ab30637, Abcam), goat anti-Golgin245 (1:500, Golgin245, DSHB), mouse anti-Cnx99A (1:200, Cnx99A 6-2-1, DSHB), mouse antiGolgin84 (1:200, Golgin84 12-1, DSHB), chicken anti-GFP (1:500, ab13970, Abcam), rabbit anti-Ifc (1:200, Jung et al, 2017) rabbit anti-Calnexin (1:200, ab75801, Abcam), rabbit anti-rab5 (1:200, ab31261, Abcam), diluted in 0.25% PBST with 1% BSA overnight at 4 °C, washed in 1% PBST four times for 30 min, and incubated in secondary antibody (1:500, 111-605-003, 715-545-150, 711-545- 152, 112-545-167, 111-165-003, 115-605-166, Jackson ImmunoResearch Laboratories) diluted in 0.25% PBST with 1% BSA overnight at 4 °C. .. The brains were then washed with 1% PBST four times for 30 min and mounted in Vectashield (H-1000, Vector Laboratories) or RapiClear 1.47 (#RC147002, SunJin Lab) for subsequent confocal imaging.



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    anti disc large dlg mouse monoclonal antibody  (Developmental Studies Hybridoma Bank)
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    Developmental Studies Hybridoma Bank anti disc large dlg mouse monoclonal antibody
    Anti Disc Large Dlg Mouse Monoclonal Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse anti discs large dlg  (Developmental Studies Hybridoma Bank)
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    (A) Experimental design. UBQLN2 P497H was expressed pan-neuronally using elav-GAL4. Parental flies were maintained on standard food supplemented with SR-0813 (1 µM; SR-1 or 10 µM; SR-10) or left untreated (UNT). Progeny were assessed at the third-instar larval stage (L3) by crawling assays, and adults were assayed for climbing performance every 5 days from day 5 to day 30 post - eclosion (D5-D30). (B–E) Larval crawling behavior. (B) Representative 30-s locomotor tracks from L3 foraging larvae (UNT, SR-1, SR-10). Quantification of (C) track length, (D) distance traveled, and (E) average speed over 30 s. Each point represents one larva (UNT n = 60; SR-1 n = 53; SR-10 n = 57). Statistical analysis: ordinary one-way ANOVA with Dunnett’s multiple-comparisons test (each SR-0813 condition versus UNT); p < 0.05 was considered significant. (F–H) Neuromuscular junction (NMJ) morphology. (F) Representative images of type Ib NMJs on muscle 4 (abdominal segment A4) from L3 elav>UBQLN2 P497H larvae reared on UNT, SR-1, or SR-10 food. Presynaptic terminals were labeled with anti-HRP (green) and postsynaptic densities <t>with</t> <t>anti-Discs</t> large (Dlg; red). Quantification of (G) NMJ branch length and (H) bouton number. Each point represents one larva (n = 30 larvae per condition). Statistical analysis: ordinary one-way ANOVA with Dunnett’s multiple-comparisons test (each SR-0813 condition versus UNT); p < 0.05 was considered significant.
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    (A) Experimental design. UBQLN2 P497H was expressed pan-neuronally using elav-GAL4. Parental flies were maintained on standard food supplemented with SR-0813 (1 µM; SR-1 or 10 µM; SR-10) or left untreated (UNT). Progeny were assessed at the third-instar larval stage (L3) by crawling assays, and adults were assayed for climbing performance every 5 days from day 5 to day 30 post - eclosion (D5-D30). (B–E) Larval crawling behavior. (B) Representative 30-s locomotor tracks from L3 foraging larvae (UNT, SR-1, SR-10). Quantification of (C) track length, (D) distance traveled, and (E) average speed over 30 s. Each point represents one larva (UNT n = 60; SR-1 n = 53; SR-10 n = 57). Statistical analysis: ordinary one-way ANOVA with Dunnett’s multiple-comparisons test (each SR-0813 condition versus UNT); p < 0.05 was considered significant. (F–H) Neuromuscular junction (NMJ) morphology. (F) Representative images of type Ib NMJs on muscle 4 (abdominal segment A4) from L3 elav>UBQLN2 P497H larvae reared on UNT, SR-1, or SR-10 food. Presynaptic terminals were labeled with anti-HRP (green) and postsynaptic densities <t>with</t> <t>anti-Discs</t> large (Dlg; red). Quantification of (G) NMJ branch length and (H) bouton number. Each point represents one larva (n = 30 larvae per condition). Statistical analysis: ordinary one-way ANOVA with Dunnett’s multiple-comparisons test (each SR-0813 condition versus UNT); p < 0.05 was considered significant.
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    (A) Experimental design. UBQLN2 P497H was expressed pan-neuronally using elav-GAL4. Parental flies were maintained on standard food supplemented with SR-0813 (1 µM; SR-1 or 10 µM; SR-10) or left untreated (UNT). Progeny were assessed at the third-instar larval stage (L3) by crawling assays, and adults were assayed for climbing performance every 5 days from day 5 to day 30 post - eclosion (D5-D30). (B–E) Larval crawling behavior. (B) Representative 30-s locomotor tracks from L3 foraging larvae (UNT, SR-1, SR-10). Quantification of (C) track length, (D) distance traveled, and (E) average speed over 30 s. Each point represents one larva (UNT n = 60; SR-1 n = 53; SR-10 n = 57). Statistical analysis: ordinary one-way ANOVA with Dunnett’s multiple-comparisons test (each SR-0813 condition versus UNT); p < 0.05 was considered significant. (F–H) Neuromuscular junction (NMJ) morphology. (F) Representative images of type Ib NMJs on muscle 4 (abdominal segment A4) from L3 elav>UBQLN2 P497H larvae reared on UNT, SR-1, or SR-10 food. Presynaptic terminals were labeled with anti-HRP (green) and postsynaptic densities <t>with</t> <t>anti-Discs</t> large (Dlg; red). Quantification of (G) NMJ branch length and (H) bouton number. Each point represents one larva (n = 30 larvae per condition). Statistical analysis: ordinary one-way ANOVA with Dunnett’s multiple-comparisons test (each SR-0813 condition versus UNT); p < 0.05 was considered significant.
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    (A) Experimental design. UBQLN2 P497H was expressed pan-neuronally using elav-GAL4. Parental flies were maintained on standard food supplemented with SR-0813 (1 µM; SR-1 or 10 µM; SR-10) or left untreated (UNT). Progeny were assessed at the third-instar larval stage (L3) by crawling assays, and adults were assayed for climbing performance every 5 days from day 5 to day 30 post - eclosion (D5-D30). (B–E) Larval crawling behavior. (B) Representative 30-s locomotor tracks from L3 foraging larvae (UNT, SR-1, SR-10). Quantification of (C) track length, (D) distance traveled, and (E) average speed over 30 s. Each point represents one larva (UNT n = 60; SR-1 n = 53; SR-10 n = 57). Statistical analysis: ordinary one-way ANOVA with Dunnett’s multiple-comparisons test (each SR-0813 condition versus UNT); p < 0.05 was considered significant. (F–H) Neuromuscular junction (NMJ) morphology. (F) Representative images of type Ib NMJs on muscle 4 (abdominal segment A4) from L3 elav>UBQLN2 P497H larvae reared on UNT, SR-1, or SR-10 food. Presynaptic terminals were labeled with anti-HRP (green) and postsynaptic densities <t>with</t> <t>anti-Discs</t> large (Dlg; red). Quantification of (G) NMJ branch length and (H) bouton number. Each point represents one larva (n = 30 larvae per condition). Statistical analysis: ordinary one-way ANOVA with Dunnett’s multiple-comparisons test (each SR-0813 condition versus UNT); p < 0.05 was considered significant.
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    (A) Brightfield (BF) images of third instar larval salivary glands from control ( w;;; ) and double mutant ( w; Sep5²; Sep2² ) larvae. Salivary glands of double mutants appear morphologically different, with signs of tissue melanization (black area). (B) Confocal images of salivary glands stained with DAPI (blue; nuclei), anti-E-cadherin (green; E-cad), <t>and</t> <t>anti-DLG1</t> (red; Discs Large) in control and double mutant larvae. Note the irregular shape and size of the salivary gland and its defective tubular structure, including the disorganized structure of the cell borders and nuclei. (C) High-magnification images of the epithelial cell layer show defects in the localization and distribution of E-cadherin and DLG1 in salivary glands of double mutants. Nuclear positioning, shape, and size are also defective in double mutants. Scale bars: 20 μm (all panels).
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    mouse monoclonal anti dlg1  (Developmental Studies Hybridoma Bank)
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    Developmental Studies Hybridoma Bank mouse monoclonal anti dlg1
    (A) Brightfield (BF) images of third instar larval salivary glands from control ( w;;; ) and double mutant ( w; Sep5²; Sep2² ) larvae. Salivary glands of double mutants appear morphologically different, with signs of tissue melanization (black area). (B) Confocal images of salivary glands stained with DAPI (blue; nuclei), anti-E-cadherin (green; E-cad), <t>and</t> <t>anti-DLG1</t> (red; Discs Large) in control and double mutant larvae. Note the irregular shape and size of the salivary gland and its defective tubular structure, including the disorganized structure of the cell borders and nuclei. (C) High-magnification images of the epithelial cell layer show defects in the localization and distribution of E-cadherin and DLG1 in salivary glands of double mutants. Nuclear positioning, shape, and size are also defective in double mutants. Scale bars: 20 μm (all panels).
    Mouse Monoclonal Anti Dlg1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse α dlg1  (Developmental Studies Hybridoma Bank)
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    Developmental Studies Hybridoma Bank mouse α dlg1
    (A) Brightfield (BF) images of third instar larval salivary glands from control ( w;;; ) and double mutant ( w; Sep5²; Sep2² ) larvae. Salivary glands of double mutants appear morphologically different, with signs of tissue melanization (black area). (B) Confocal images of salivary glands stained with DAPI (blue; nuclei), anti-E-cadherin (green; E-cad), <t>and</t> <t>anti-DLG1</t> (red; Discs Large) in control and double mutant larvae. Note the irregular shape and size of the salivary gland and its defective tubular structure, including the disorganized structure of the cell borders and nuclei. (C) High-magnification images of the epithelial cell layer show defects in the localization and distribution of E-cadherin and DLG1 in salivary glands of double mutants. Nuclear positioning, shape, and size are also defective in double mutants. Scale bars: 20 μm (all panels).
    Mouse α Dlg1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+dlg/anti-DLG-1/bio_rxiv__64898__2026__01__08__698469-184-21-23
    Average 96 stars, based on 1 article reviews
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    Image Search Results


    (A) Experimental design. UBQLN2 P497H was expressed pan-neuronally using elav-GAL4. Parental flies were maintained on standard food supplemented with SR-0813 (1 µM; SR-1 or 10 µM; SR-10) or left untreated (UNT). Progeny were assessed at the third-instar larval stage (L3) by crawling assays, and adults were assayed for climbing performance every 5 days from day 5 to day 30 post - eclosion (D5-D30). (B–E) Larval crawling behavior. (B) Representative 30-s locomotor tracks from L3 foraging larvae (UNT, SR-1, SR-10). Quantification of (C) track length, (D) distance traveled, and (E) average speed over 30 s. Each point represents one larva (UNT n = 60; SR-1 n = 53; SR-10 n = 57). Statistical analysis: ordinary one-way ANOVA with Dunnett’s multiple-comparisons test (each SR-0813 condition versus UNT); p < 0.05 was considered significant. (F–H) Neuromuscular junction (NMJ) morphology. (F) Representative images of type Ib NMJs on muscle 4 (abdominal segment A4) from L3 elav>UBQLN2 P497H larvae reared on UNT, SR-1, or SR-10 food. Presynaptic terminals were labeled with anti-HRP (green) and postsynaptic densities with anti-Discs large (Dlg; red). Quantification of (G) NMJ branch length and (H) bouton number. Each point represents one larva (n = 30 larvae per condition). Statistical analysis: ordinary one-way ANOVA with Dunnett’s multiple-comparisons test (each SR-0813 condition versus UNT); p < 0.05 was considered significant.

    Journal: bioRxiv

    Article Title: A leukemia-derived ENL/AF9 chemical probe enhances neuronal stress resilience and ameliorates ALS phenotypes

    doi: 10.64898/2026.04.09.717610

    Figure Lengend Snippet: (A) Experimental design. UBQLN2 P497H was expressed pan-neuronally using elav-GAL4. Parental flies were maintained on standard food supplemented with SR-0813 (1 µM; SR-1 or 10 µM; SR-10) or left untreated (UNT). Progeny were assessed at the third-instar larval stage (L3) by crawling assays, and adults were assayed for climbing performance every 5 days from day 5 to day 30 post - eclosion (D5-D30). (B–E) Larval crawling behavior. (B) Representative 30-s locomotor tracks from L3 foraging larvae (UNT, SR-1, SR-10). Quantification of (C) track length, (D) distance traveled, and (E) average speed over 30 s. Each point represents one larva (UNT n = 60; SR-1 n = 53; SR-10 n = 57). Statistical analysis: ordinary one-way ANOVA with Dunnett’s multiple-comparisons test (each SR-0813 condition versus UNT); p < 0.05 was considered significant. (F–H) Neuromuscular junction (NMJ) morphology. (F) Representative images of type Ib NMJs on muscle 4 (abdominal segment A4) from L3 elav>UBQLN2 P497H larvae reared on UNT, SR-1, or SR-10 food. Presynaptic terminals were labeled with anti-HRP (green) and postsynaptic densities with anti-Discs large (Dlg; red). Quantification of (G) NMJ branch length and (H) bouton number. Each point represents one larva (n = 30 larvae per condition). Statistical analysis: ordinary one-way ANOVA with Dunnett’s multiple-comparisons test (each SR-0813 condition versus UNT); p < 0.05 was considered significant.

    Article Snippet: For immunostaining, neuromuscular junctions were labeled using FITC-conjugated goat anti-horseradish peroxidase (HRP) antibody (1:1000, Jackson ImmunoResearch), which marks neuronal membranes, and mouse anti-Discs large (Dlg) antibody (1:300, clone 4F3, DSHB).

    Techniques: Labeling

    (A) Brightfield (BF) images of third instar larval salivary glands from control ( w;;; ) and double mutant ( w; Sep5²; Sep2² ) larvae. Salivary glands of double mutants appear morphologically different, with signs of tissue melanization (black area). (B) Confocal images of salivary glands stained with DAPI (blue; nuclei), anti-E-cadherin (green; E-cad), and anti-DLG1 (red; Discs Large) in control and double mutant larvae. Note the irregular shape and size of the salivary gland and its defective tubular structure, including the disorganized structure of the cell borders and nuclei. (C) High-magnification images of the epithelial cell layer show defects in the localization and distribution of E-cadherin and DLG1 in salivary glands of double mutants. Nuclear positioning, shape, and size are also defective in double mutants. Scale bars: 20 μm (all panels).

    Journal: bioRxiv

    Article Title: Septin Complexes Regulate Microtubule Organization and Synaptic Function at the Neuromuscular Junction

    doi: 10.64898/2026.03.01.708893

    Figure Lengend Snippet: (A) Brightfield (BF) images of third instar larval salivary glands from control ( w;;; ) and double mutant ( w; Sep5²; Sep2² ) larvae. Salivary glands of double mutants appear morphologically different, with signs of tissue melanization (black area). (B) Confocal images of salivary glands stained with DAPI (blue; nuclei), anti-E-cadherin (green; E-cad), and anti-DLG1 (red; Discs Large) in control and double mutant larvae. Note the irregular shape and size of the salivary gland and its defective tubular structure, including the disorganized structure of the cell borders and nuclei. (C) High-magnification images of the epithelial cell layer show defects in the localization and distribution of E-cadherin and DLG1 in salivary glands of double mutants. Nuclear positioning, shape, and size are also defective in double mutants. Scale bars: 20 μm (all panels).

    Article Snippet: Rabbit anti-HRP (Jackson ImmunoResearch Labs Cat# 323-005-021, RRID: AB_2314648) (1:2000 dilution), 4F3 mouse anti-DLG1 (AB_528203, DSHB) (1:50 dilution), Mouse anti-Syn (AB_528479, DSHB) (1:25 dilution), Mouse anti-BRP (AB_2314866, DSHB) (1:100 dilution), Mouse anti-Futsch (AB_528403, DSHB) (1:50 dilution), Mouse anti-GluRAII (AB_528269, DSHB) (1:50 dilution).

    Techniques: Control, Mutagenesis, Staining